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KMID : 1024520190280020249
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Journal of the Environmental Sciences
2019 Volume.28 No. 2 p.249 ~ p.257
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Effects of a Glycoprotein Isolated from Ulmus davidiana Nakai on Toluene-Induced Ecotoxicity and its Mechanism in Human Intestinal Epithelial Cells
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Kim Do-Wan
Kim Ji-Yun
Park Moon-Ki
Lee Sei-Jung
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Abstract
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Ulmus davidiana Nakai (UDN) has been traditionally used as a herbal medicine to treat inflammatory diseases in Korea. In the present study, we investigated the anti-ecotoxic potential of a 116 kDa glycoprotein isolated from UDN (UDN glycoprotein) in human intestinal epithelial INT-407 cells. We demonstrated that UDN glycoprotein (20 μg/mL) could inhibit the production of lactate dehydrogenase (LDH) induced by toluene, an ecotoxic substance. Additionally, we found that the toluene-induced intestinal cytotoxicity was mediated by the phosphorylation of p38 Mitogen-Activated Protein Kinase (MAPK) via the production of intracellular Reactive Oxygen Species (ROS). The UDN glycoprotein significantly decreased the levels of ROS production and p38 MAPK activation in toluene-stimulated INT-407 cells. Moreover, the UDN glycoprotein inhibits the phosphorylation of nuclear factor-kappa B (NF-κB), which is responsible for the production of LDH, in toluene-stimulated INT-407 cells. Collectively, our data indicate that UDN glycoprotein is a natural antioxidant and a modulator of ecotoxicity signaling pathways in human intestinal epithelial cells.
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KEYWORD
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Ulmus davidiana Nakai (UDN) glycoprotein, Intestinal epithelial cells, Reactive oxygen species, NF-κB, Toluene
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